Various studies have measured accommodative microfluctuations (AMF)1,2, and Critical Flicker Fusion Frequency (CFFF)3,4 for visual fatigue assessment. Also, several products are available these days claiming relief from eyestrain mostly either by relaxing accommodation or enhancing contrast. Apart from AMF, either CFFF or contrast measurements are not well established for visual fatigue assessment. Therefore, this study aims to measure three distinct parameters: AMF, Contrast Sensitivity (CS) and CFFF in one study; to compare their efficacy for the assessment of visual fatigue.